Description
Recombinant human fusion protein TECR (1-5a.a., exons1)-PKN1 (473a.a.-end, exons10-22) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number of TECR is NM_138501, and the gene accession number of PKN1 is NM_002741.
PKN1/PRK1 belongs to the protein kinase C superfamily which is activated by Rho family of small G proteins. PKN1/PRK1 is known to mediate the Rho-dependent signaling pathway and it can be activated by phospholipids and by limited proteolysis (1). PDPK1/PDK may also mediate insulin signals to the actin cytoskeleton and the proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggest its role in signal transduction related to apoptosis. PKN1/PRK signaling stimulates AR activity in the presence of adrenal androgens and in the presence of an AR antagonist (2)